Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: PRDX6 Protects ARPE-19 Cells from Oxidative Damage via PI3K/AKT Signaling.

doi: 10.1159/000430186

Figure Lengend Snippet: Fig. 3. PRDX6 protected ARPE-19 cells from undergoing oxidative stress and cell death induced by H2O2 and blue light. ARPE-19 cells were transfected with the PRDX6-pSilencer 4.1 plasmid or the PRDX6-pcD NA3.1 overexpression plasmid, and the cells were allowed to recover in regular culture medium. At 24 h post-transfection, the cells were exposed to 0.5 or 1.0 mM H2O2 and blue light for 24 h. (A, B) Apoptosis (A) and necrosis (B) were determined by annexin V/PI double staining, followed by flow cytometry. (C) Cells were treated as above. After immunostaining, the green fluorescence of JC-1 monomer and red fluorescence of J-aggregates were clustered in groups. (D) MDA concentration in cells was detected by MDA assay. (E) 8-OHdG concentration in cells was tested by 8-OHdG assay. (F) The cells were stained with DCF-DA to detect intracellular ROS production. The values represent the mean ± S.D. of three independent experiments. Stati stical significance was assessed using one-way ANOVA plus Tukey’s test using GraphPad Prism Version 5.0a software (*p < 0.05; **p < 0.01; ***p < 0.001 vs. control).

Article Snippet: Apoptosis was quantified using an Annexin V-FITC Apoptosis Detection Kit (Beyotime, Nantong, China).

Techniques: Transfection, Plasmid Preparation, Over Expression, Double Staining, Flow Cytometry, Immunostaining, Fluorescence, Concentration Assay, Multiple Displacement Amplification, Staining, Software, Control

Journal: International journal of oral science

Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.

doi: 10.1038/s41368-022-00192-2

Figure Lengend Snippet: Fig. 2 OSCC-derived sEVs enhance the proliferation and inhibit the apoptosis of normal epithelial cells. a, b The proliferative cells of HIOECs treated with OSCC-derived sEVs. Scale bar, 50 μm. c The size and morphology of HIOECs in Matrigel treated with OSCC-derived sEVs. Scale bar, 50 μm. d Immunofluorescence staining of Ki67 in HIOECs treated with OSCC-derived sEVs in Matrigel. Scale bars, 100 μm. e, f The apoptosis rate of HIOECs treated with OSCC-derived sEVs. g The expression of apoptosis-related markers in HIOECs treated with OSCC-derived sEVs

Article Snippet: EdU assay The assay was carried out as previously described.43 In summary, HIOECs were uniformly plated into 24-well plates, fixed and stained using the EdU Cell Proliferation Kit (Beyotime, China) as instructed, and finally observed and photographed under a fluorescence microscope (Keyence, Japan).

Techniques: Derivative Assay, Staining, Expressing

Journal: International journal of oral science

Article Title: Cancer cells corrupt normal epithelial cells through miR-let-7c-rich small extracellular vesicle-mediated downregulation of p53/PTEN.

doi: 10.1038/s41368-022-00192-2

Figure Lengend Snippet: Fig. 5 MiRNA let-7c plays a similar role to OSCC-derived sEVs on normal epithelial cells. a The proliferation ability of HIOECs transfected with let-7c mimics. b The proliferative cells of HIOECs transfected with let-7c mimics. c The size and morphology of HIOECs transfected with let-7c mimics in Matrigel. Scale bar, 50 μm. d Immunofluorescence staining of Ki67 in HIOECs transfected with let-7c mimics in Matrigel. Scale bars, 100 μm. e, f The apoptosis rate of HIOECs transfected with let-7c mimics. g The expression of apoptosis-related markers in HIOECs transfected with let-7c mimics. h The expression of EMT-related markers in HIOECs transfected with let-7c mimics

Article Snippet: EdU assay The assay was carried out as previously described.43 In summary, HIOECs were uniformly plated into 24-well plates, fixed and stained using the EdU Cell Proliferation Kit (Beyotime, China) as instructed, and finally observed and photographed under a fluorescence microscope (Keyence, Japan).

Techniques: Derivative Assay, Transfection, Staining, Expressing